TABLE 2.
Antigen binding specificities of JR-FL SOSIP-specific MAbs
| MAb | Bindinga |
Specificity | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JR-FL gp140-F | JR-FL SOSIP | gp120 |
V3 peptideb |
||||||||||
| ΔV1V2 | Core | WT | I420R | D368R | ΔV3 | F4 | F5 | F6 | FL | ||||
| S1 | NT | ++ | ++++ | ++++ | ++ | + | ++++ | ++++ | − | +++ | V3 | ||
| S7 | ++++ | + | + | + | + | − | − | − | − | − | V3 dependent | ||
| S12 | ++ | ++ | ++++ | ++++ | + | ++++ | − | − | − | − | CD4bs | ||
| S13 | ++ | ++ | +++ | − | ++++ | ++++ | ++++ | ++++ | − | − | − | − | V1V2 |
| S15 | ++ | ++ | ++++ | ++++ | ++++ | + | + | +++ | − | +++ | V3 | ||
| S18 | ++++ | +++ | ++++ | ++++ | ++++ | − | ++ | +++ | − | +++ | V3 | ||
| S25 | NT | ++++ | NT | NT | NT | NT | +++ | ++++ | + | ++++ | V3 | ||
| S30 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | + | ++++ | V3 | ||
| S33 | ++ | + | ++ | + | ++ | − | − | − | − | − | V3 dependent | ||
| S44 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | + | ++++ | V3 | ||
| S48 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | + | ++++ | V3 | ||
| S54 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | + | ++++ | V3 | ||
| S57 | ++++ | + | ++++ | ++++ | ++++ | − | − | +++ | − | +++ | V3 | ||
| S62 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | +++ | − | +++ | V3 | ||
| S68 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | − | ++++ | V3 | ||
| S72 | ++++ | +++ | ++++ | ++++ | ++++ | − | − | ++ | − | ++++ | V3 | ||
| S74 | ++ | ++ | ++++ | ++++ | ++++ | + | − | + | − | +++ | V3 | ||
| S75 | ++ | + | ++++ | +++ | ++++ | ++++ | ++++ | ++++ | − | − | − | − | Core |
| S76 | ++ | ++ | ++++ | ++++ | ++++ | +++ | ++++ | ++++ | − | ++++ | V3 | ||
| S80 | ++++ | +++ | ++++ | ++++ | ++++ | − | − | +++ | +++ | ++++ | V3 | ||
| S81 | NT | ++++ | NT | NT | NT | NT | +++ | ++++ | + | ++++ | V3 | ||
| S87 | ++ | ++ | ++++ | ++++ | ++ | ++++ | − | − | − | − | CD4bs | ||
| S89 | ++++ | +++ | ++ | ++++ | ++++ | − | + | + | − | +++ | V3 | ||
| S96 | ++++ | +++ | ++++ | ++++ | ++++ | − | − | +++ | +++ | ++++ | V3 | ||
| S98 | ++ | ++++ | ++++ | ++++ | ++++ | + | ++++ | +++ | + | ++++ | V3 | ||
| S100 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | + | ++++ | V3 | ||
| S102 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | + | ++++ | V3 | ||
| S105 | ++ | ++ | ++++ | ++++ | ++ | − | +++ | ++++ | + | +++ | V3 | ||
| S109 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | + | ++++ | V3 | ||
| S110 | + | + | + | + | + | + | NT | NT | NT | NT | unknown | ||
| S118 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | + | ++++ | V3 | ||
| S119 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | ++++ | + | +++ | V3 | ||
| S124 | ++++ | +++ | ++ | ++++ | ++++ | − | − | +++ | − | ++++ | V3 | ||
| S128 | ++++ | ++ | ++++ | ++++ | ++++ | − | +++ | +++ | + | +++ | V3 | ||
| S134 | ++ | ++ | ++++ | ++++ | ++++ | − | +++ | ++++ | − | ++++ | V3 | ||
| S138 | ++ | ++ | ++++ | ++++ | ++++ | − | ++++ | +++ | + | +++ | V3 | ||
| S146 | + | + | + | + | + | + | NT | NT | NT | NT | unknown | ||
| S147 | ++ | + | ++++ | ++++ | ++++ | − | + | + | − | +++ | V3 | ||
| S152 | ++ | ++ | ++++ | ++++ | ++++ | − | + | ++++ | + | ++++ | V3 | ||
a
++++, OD450 ≥ 3 and EC50 ≤ 0.1; +++, OD450 ≥ 3 and EC50 > 0.1; ++, 3 > OD450 ≥ 1; +, 1.0 > OD450 ≥ 0.2; −, OD450 < 0.2; NT, not tested.
b
FL, full-length V3; F4, F5, and F6, fragments 4 to 6, respectively.