FIG 5.

Human IAV NS1 p85β-binding variants have functional consequences. (A) Western blot analysis of anti-V5 immunoprecipitates from lysates of 293T cells expressing V5-GST or various V5-NS1 mutants together with FLAG-p85β. (B) Same as panel A, but including V5-NS1 proteins derived from the indicated virus strains. The sequences at key residues are indicated at the top. For both panel A and panel B, proteins of interest were detected using specific antibodies. Input samples were similarly processed. Band intensity was quantified using a Li-Cor Odyssey scanner. The mean numbers of relative units from three independent experiments are plotted. Error bars represent standard deviations (SDs). Significance was determined by the Student t test (*, P < 0.05).