FIG 7.

Impact of NS1 p85β-binding variants on IAV-induced Akt phosphorylation and replication in vitro. (A) Western blot analysis of total lysates of HAP1 cells infected with the indicated rPR8-based NS1 wild-type (WT) or mutant virus at an MOI of 5 PFU/cell for 2, 4, or 6 h. Proteins of interest were detected using specific antibodies. Data are representative of those from at least three independent experiments. VT, L95V/I145T. (B and C) Growth curve analysis of the indicated rPR8-based NS1 wild-type or mutant virus in A549 cells (MOI = 0.01 PFU/cell). Supernatants from the indicated time points were titrated in MDCK cells. Data points show mean values from three independent experiments ± SDs.