FIG 8.

Impact of NS1 p85β-binding variants on IAV fitness in vitro. A549 cells were initially infected with the indicated mixture of rPR8-based NS1 wild-type (WT) or mutant virus at an MOI of 0.01 PFU/cell. For each passage, supernatants were harvested after 48 h, titrated, and used for subsequent passages at the same MOI. The graphs depict the relative prevalence of the indicated segment at each passage (P), as determined by Sanger sequencing and analysis of the relevant codon. Each point represents data from a single infected well of a single experiment (3 wells in each of 2 independent experiments). Rel. seq., relative sequence; VT, L95V/I145T.