FIG 2.

K15P-HA does not colocalize with lysosomal or mitochondrial markers in a density fractionation gradient. The PNS from BCBL-1-HA cells was loaded at the top of a 2.5% to 30% iodixanol gradient and subjected to ultracentrifugation and then SDS-PAGE and Western blotting of fractions from top (fraction 1) to bottom (fraction 24). Blots were probed with antibodies against K15P-HA (HA), the endoplasmic reticulum (PDI), the Golgi apparatus (p58K), the plasma membrane (Na/K [Na⁺/K⁺ ATPase]), mitochondria (SMAC), and lysosomes (CB [cathepsin B]), as indicated at right. Positions of molecular size markers are indicated at left.