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. 2017 Sep;6(3):101–112. doi: 10.22099/mbrc.2017.4081

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression, Western blot analysis and purification of recombinant CII. A) Optimization of recombinant CII (lanes 2-6) with 1mM IPTG. Lane 7, protein weight marker. Lane 1, uninduced E. coli BL21DE3/pET32a + cII gene as control. B) Purification of recombinant CII protein with 6X-His-tagged from pET32.Lane1, protein weight marker. Lane 2, flow-through. Lane 3, wash column with 40mM imidazole, Lanes 4-6, purified protein after elution with 250mM imidazole. C) Western blot analysis of CII using anti 6X-His-tag antibodies. Lane 1, protein weight marker. Lane 2, CII. Lane 3, total protein of E. coli BL21DE3/pET32 without cII gene after induction as control