Fig. 2.

H₂S-induced H₂O₂ accumulation is diminished by the scavenger and synthetic inhibitor of H₂O₂. Three-day-old tomato seedlings were treated with H₂O (Con), 1 mM NaHS, 100 μΜ H₂O₂, 500 μΜ N,N′-dimethylthiourea (DMTU), and 0.1 μΜ diphenylene idonium (DPI), alone or their combinations for 12 h. Afterwards, corresponding confocal images of DCF-dependent fluorescence in seedling roots were provided to represent endogenous H₂O₂ contents (a), and the relative fluorescence were presented as values relative to Con (b). Scale bar = 200 μm. Meanwhile, the H₂O₂ contents were determined by spectrophotography (c). Mean and SE values were calculated from at least three independent experiments. Bars with different letters denoted significant differences at P < 0.05 according to Duncan’s multiple range test