Fig. 7.

H₂S affects the expression of microRNAs and their target genes. Three-day-old tomato seedlings were treated with H₂O (Con), 1 mM NaHS, 100 μΜ H₂O₂, 500 μΜ N,N′-dimethylthiourea (DMTU), and 0.1 μΜ diphenylene idonium (DPI), alone or their combinations for 12 h. Meanwhile, miR390a (a; black), SlARF4 (a; white), miR160 (b; black), and SlARF16 (b; white) transcript levels were analyzed by qPCR, and presented relative to the Con. Mean and SE values were calculated from at least three independent experiments. Within each set of experiments, bars with different letters denoted significant differences at P < 0.05 according to Duncan’s multiple range test