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. 2017 Oct 13;13:83. doi: 10.1186/s13007-017-0231-1

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Examples of over counting (a, b) and undercounting(c, d) problems: a the misclassification of a leaf as a spike; b the miscount of one spike as two spikes; c the miscount of two overlapping spikes as a single spike; d a young spike not classified as a spike