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. Author manuscript; available in PMC: 2017 Oct 14.
Published in final edited form as: J Alcohol Drug Depend. 2017 Jul 20;5(4):273. doi: 10.4172/2329-6488.1000273

Figure 2.

Figure 2

Double immunofluorescence staining demonstrating the location of orexin (Orex) immunoreactive (IR) nerve fibers with respect to nicotine (NIC) induced c-Fos IR cells of lateral hypothalamus (LH). Panel A: Low power fluorescent image showing NIC-induced c-Fos IR and Orex IR nerve fibers in LH Panels B-D: High power images showing NIC-induced c-Fos IR cells (B), Orex IR nerve fibers (C) and merged images of c-Fos with Orex IR fibers (D). Orex IR nerve fibers are seen in areas overlapping NIC induced c-Fos activated cells of LH and several other hypothalamic regions. Solid arrows point to representative c-Fos IR and broken arrows to orexin IR nerve fibers. Square indicates magnified area (3V 3rd Ventricle).