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. Author manuscript; available in PMC: 2018 Apr 4.
Published in final edited form as: Nature. 2017 Oct 4;550(7675):265–269. doi: 10.1038/nature24021

Extended data Figure 2. TORC1 focus formation occurs upon glucose-starvation but not nitrogen or leucine starvation independently of external pH.

Extended data Figure 2

a, The majority of cells grown into stationary phase display a prominent TORC1 focus as determined by confocal microscopy imaging of GFP-Kog1. Addition of glucose, but not amino acids (AA) or ammonium sulphate (AS) triggered rapid disassembly of these foci. b, TORC1 focus formation monitored by confocal microscopy using a microfluidic device demonstrates that foci can be observed within 2-3 minutes after glucose depletion. c-e, TORC1 focus formation monitored by confocal microscopy after Glucose (c), Ammonium sulphate (d), or Leucine (e) starvation and subsequent re-addition. f, Confocal images of WT cells expressing GFP-Kog1 and Gln1-mCherry grown in pH-controlled medium before (+Glucose) and 1 hour after (-Glucose) glucose starvation. White arrows show Gln1 foci.