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. 2017 May 22;8(42):71400–71417. doi: 10.18632/oncotarget.18069

Figure 1. Endometrial cancer cells form distinct glandular and non-glandular pattern in reconstituted basement membrane and with endometrial stromal fibroblast co-culture.

Figure 1

A. Ishikawa and MFE-296 cells were grown as monolayers (top row), spheroids without ECM (middle row) and spheroids with ECM (bottom row), Phase contrast scale bar, 50 μm. B. Cells were cultured in RGF-BME for 7 days, stained for pan-cytokeratin (green), phalloidin (red), counterstained with Hoechst (blue), and imaged by confocal microscopy. One representative confocal section is shown out of 100 similar colonies. Phase contrast scale bar, 50 μm; confocal scale bar, 10 μm, Phase contrast scale bar, 50 μm. C. Comparison of cell proliferation and colony diameter between adherent (left) and spheroid (right) culture (n = 3). D. Fluorescence and phase contrast images of endometrial epithelial and fibroblast co-culture. Ep: Epithelial cells, St: Stromal cells. Scale bar, 200 μm. Error bars represent mean ± SD; ns = P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.