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. 2017 Mar 13;8(42):71447–71455. doi: 10.18632/oncotarget.16169

Figure 1. CTC capture and validation in LNCAP and mCRPC patients.

Figure 1

(A) Flowchart of CTC isolation, detection and molecular analysis workflow. (B) Left panel: LNCAP cells spiked into blood as a model for CTC capture and assay validation; Middle panel: Patient CTC without AR amplification; Right panel: Patient CTC with AR amplification. Upper panel immunofluorescence: Blue: dapi, Green: CK20, Pink: CD45. Lower panel: AR FISH analysis of cell identified in upper panel. Red: AR signal, green: X CEP control signal.