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. 2017 Apr 20;8(42):71512–71519. doi: 10.18632/oncotarget.17292

Figure 2. Albendazole caused cell death and apoptosis.

Figure 2

(A) Live/dead assays were performed with Cal33, HSC2, SCC47, 93-VU-147T and WI38 cell samples that were exposed to vehicle (DMSO-only) or 0.5 μM of albendazole (ABZ) for 24 hours (four replicates per treatment). Albendazole treatment significantly increased the percentage of dead cells in susceptible cell lines (Cal33 and SCC47; paired t-test, p = 0.026 and p < 0.001 respectively), but yielded insignificant changes in HSC2, 93-VU-147T and WI38 cell lines. “(S)” marks the susceptible, and “(R)” marks the non-susceptible cell lines by IC50 analysis. Error bars show standard error, *p < 0.05 and **p < 0.01. (B) Cal33 cells were exposed to vehicle (DMSO-only), 0.5 μM or 1 μM ABZ for 24 hours, and immunoblotted for PARP. Staurosporine (St.) treatment was used as a positive control for apoptosis. Presence of cleaved PARP (c-PARP) at 1 μM albendazole treatment suggested that apoptosis was involved in cell death.