Figure 4. IL-35 suppresses colon tumorigenesis in vivo.

The adeno-associated virus expressing IL-35 or GFP was administrated via tail-vein injection. The average tumor number per mice (A) and the tumor size (B) were analyzed. Western blot showed that IL-35 suppressed β-catenin expression in the mice tumor (C). Tumor cells were isolated from the mice and then subjected to chemotherapeutic drugs treatment (D). n = 12. *P < 0.05.