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. 2017 May 11;8(42):71630–71641. doi: 10.18632/oncotarget.17795

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Copyright: © 2017 Luo et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A–C) Western blot analysis of MGr1-Ag/37LRP expression in PrP^C up-regulated and down-regulated gastric cancer cells. PrP^C expression in SGC7901/PrP (lane 2) increased significantly compared to SGC7901/PCDNA3.1 (lane 3) (*p < 0.05 in B) and decreased in SGC7901/PrPi (lane 4) compared to SGC7901/Psilencer3.1 (lane 5) (**p < 0.05 in B). MGr1-Ag/37LRP protein expression was also higher in SGC7901/PrP and lower in SGC7901/PrPi (A&C). SGC7901/VCR was used as positive control and β-actin as internal control. (D) and (E) Western blot analysis showed that expression level of MGr1-Ag/37LRP protein was markedly suppressed in SGC7901/PrP (lane 3) and SGC7901/VCR (lane 6) compared with siRNA negative control (lane 2 and lane 4) after transiently transfection of MGr1-Ag/37LRP siRNA (**p < 0.05 in E). β-actin was used as internal control.