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. 2017 May 11;8(42):71672–71684. doi: 10.18632/oncotarget.17800

Figure 4. Conditioned medium from pancreatic stellate cells stimulates Met phosphorylation in pancreatic cancer cells.

Figure 4

(A) Conditioned medium from PSC populations SC40 and SC41 were transferred to BxPC-3 cells and incubated for 0, 3, 5 and 10 minutes. Effect of the PSCs on phosphorylation of EGFR and Met was measured by western blot and results from one representative experiment are shown. (B) The band intensity of the blots were quantified and normalized to GAPDH expression. Histograms represent mean +/−SEM of four experiments. (C) Phosphorylation of EGFR and Met was analysed by western blot after stimulating BxPC-3 cells for 0, 3, 5 and 10 minutes with EGF (10 nM) and HGF (1 nM). Results from one representative experiment are shown.