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. 2017 May 30;8(42):71845–71858. doi: 10.18632/oncotarget.18286

Figure 4. Prep1(54-72) peptide restores the insulin signaling inhibited by C2cer and Prep1.

Figure 4

L6 cells were stimulated with insulin (100nM) and incubated with Prep1(54-72) in presence of C2cer at 10μM for 18h (A-B) or stable transfection of Prep1 (C-D). Protein lysates were immunoprecipitated with specific IR or IRS1 antibodies and analyzed by Western blot using antibodies for phosphotyrosines (pY) and beta-actin as loading control. Blots were detected by ECL and autoradiography. The autoradiographs are representative of three independent experiments. Asterisks denote statistically significant differences (*p<0.05; **p<0.01).