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. 2017 May 30;8(42):71845–71858. doi: 10.18632/oncotarget.18286

Figure 5. Effect of Prep1(54-72) peptide on Akt phosphorylation and PGC-1α and GLUT4 protein levels.

Figure 5

(A-B) L6 control and Prep1 overexpressing cells were stimulated with Insulin (100nM) and/or C2cer (10μM) and incubated with Prep1(54-72). Protein lysates were analyzed by Western blot using antibodies for pAkt, Akt and for the beta-actin, as a loading control. (C) PGC-1α and GLUT4 protein levels were measured by using specific antibodies for these proteins and beta-actin antibody was used as loading control. Blots were detected by ECL and autoradiography. The autoradiographs are representative of four independent experiments. Asterisks denote statistically significant differences (*p<0.05; **p<0.01; ***p<0.001).