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. 2017 May 30;8(42):71867–71880. doi: 10.18632/oncotarget.18288

Figure 4. Barx1 inhibits HCC metastasis through the inhibition of MGAT5 and MMP9 expression.

Figure 4

(A and B) Following the infection of the SMMC7721-shBarx1 cells with the lentivirus Lenti-shMGAT5 or Lenti-shMMP9, respectively, (A) the protein levels of Barx1, MGAT5, and MMP9 were detected by western blot, and (B) the cell migration and invasion capacities were assessed using transwell assays. Data are represented as mean ± SEM for triplicate experiments. (C) Representative images of migration and invasion of the indicated cell lines. (D-H) In vivo metastatic assay. Cell lines were transplanted into the livers of nude mice. (D) Representative bioluminescent imaging (BLI) of the different groups is shown at 10 weeks following orthotopic implantation. The incidence of lung metastasis (E), overall survival (F), the number of lung metastatic foci (G), and representative H&E staining of lung tissues (H) from the different groups is shown. Scale bars represent 200 μm (low magnification) and 50 μm (high magnification). Data are represented as mean ± SEM. *P < 0.05.