Figure 6. EMC4 cell lines acquire a more anergic phenotype upon engraftment.

(A, B) Flow cytometry analysis of a-IgM-stimulated (20 μg/ml) Ca²⁺-influx between leukemic cells, directly isolated from EMC4-engrafted mice (A, grey line) or after 48 hours of culture (B, grey line), compared with in vitro cultured (non-transferred) EMC4 cells (A,B: solid black line). (C, D) Expression of surface IgM (sIgM) and cytoplasmic IgM (cIgM) between CD5⁺CD19⁺CD43⁺ splenic leukemic cells from EMC4-engrafted mice analyzed directly after isolation (C, shaded area) or after 48 hours of culture (D, shaded area), compared with an in vitro cultured (non-transferred) EMC4 cells (solid black line).