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. 2017 Jun 30;8(42):72021–72030. doi: 10.18632/oncotarget.18894

Figure 3. MARCKS promotes invasion and is associated with altered actin patterning.

Figure 3

(A) MARCKS knockdown reduces migration of the aggressive prostate cancer cell line PC3 (P≤0.001) but not the well-differentiatedPNT2 cell line. (B) MARCKS knockdown decreases invasion in both the PC3 (P≤0.001) and PNT2 (P≤0.05) cell line. (C) Representative (n=3 individual experiments) cropped western blots showing MARCKS knockdown. There is no evidence of a cadherin switch, decrease in Vinculin or a perturbance in the AKT pathway in response to MARCKS knockdown. There is decrease in FAK in the siMARCKS treated cells. (D) Immunofluorescent staining of actin (red) in response to MARCKS knockdown demonstrates less surface protrusions and cell spreading in PC3 cells. PC3 cell colonies treated with siMARCKS are more compact with more cell-cell adhesion than those treated with control (non-targeting) siRNA. (E) MMP9 gene expression is decreased in PC3 cells following MARCKS knockdown. Error bars +/− SEM. P-value calculated using Student's T-Test, at least n=3 individual experiments except for the FAK PNT-2 western which is an n=1.