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. 2017 Aug 22;8(42):72363–72374. doi: 10.18632/oncotarget.20390

Figure 4. H19/Igf2 imprinting transcription and methylation statuses and the expression of genes regulating imprinting methylation in PFFs and cloned embryos, and the cloned blastocysts derived from the abnormal imprinting PFFs with H19 knockdown.

Figure 4

(A) H19 transcription in the abnormal imprinting PFFs after siRNA transfection, (B) the methylation statuses of H19/Igf2 imprinting at the 4-cell and blastocyst stages of cloned embryos derived from the abnormal imprinting PFFs in the siRNA-control, siRNA-negative and siRNA-positive groups, (C) the transcription of H19/Igf2 and genes regulating imprinting methylation at the 4-cell and blastocyst stages of cloned embryos derived from the abnormal imprinting PFFs in the siRNA-control, siRNA-negative and siRNA-positive groups, (D) the cloned blastocysts derived from the abnormal imprinting PFFs in the siRNA-control, siRNA-negative and siRNA-positive groups (Scale bar = 500 μm). H19 knockdown in the abnormal imprinting PFFs improved H19/Igf2 imprinting methylation and transcription, the expression of genes regulating imprinting methylation and blastocyst rate. siRNA-control, siRNA-negative and siRNA-positive represented the abnormal imprinting PFFs transfected with none siRNA, negative siRNA and positive siRNA, respectively. Black or white circles indicate methylated or unmethylated CpG sites, respectively. a–cValues for a given gene with different superscripts differ significantly (P < 0.05).