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. 2017 Aug 3;8(42):72494–72512. doi: 10.18632/oncotarget.19868

Figure 5. EGFR is constitutively active in CSCs.

Figure 5

(A) J3T adherent and sphere cells were treated with 5 μM gefitinib and harvested over the indicated time course. Expression of EGFR, phosphorylation of EGFR at serine-1047 and tyrosine-1173, AKT, phosphorylation of AKT, and β-actin as a loading control. 20 μg was loaded per lane. Comparison of the effect of increasing doses of gefitinib on proliferation of adherent and spheres of J3T (B) and LN18 cells (C). Cells were treated with increasing doses of gefitinib and cell viability was assayed 48 hours post treatment.