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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Acta Physiol (Oxf). 2017 Jun 13;221(3):193–203. doi: 10.1111/apha.12897

Figure 5. SIRT1 overexpression in muscle does not alter markers of mitochondrial biogenesis.

Figure 5

(A) Transcript and (B) protein levels of PGC-1α in skeletal muscle of WT and i-mOX mice. Bar graph shows quantification of protein levels in skeletal muscle relative to eEF2 (n=7). (C) Transcript levels of mitochondrial proteins Cs, Cytc and Sdhb in skeletal muscle (n=6). (D) Representative blot of mitochondrial proteins in skeletal muscle from WT and i-mOX mice. (E) Quantification of mitochondrial protein expression in skeletal muscle relative to eEF2 (n=7). (F) Respiratory flux normalized to muscle weight in the presence of malate + octanoylcarnitine (M+Octc; leak respiration in the absence of adenylates), ADP, cytochrome c (CytC; mitochondrial integrity), pyruvate (Pyr; respiration in the presence of pyruvate, octanoylcarnitine and adenylates), glutamate (Glut; complex I (CI) capacity), succinate (Succ; complex I + complex II (CII) capacity), FCCP (maximal respiration), rotenone (Rot; complex II capacity), and Antimycin A (AmA; residual oxygen consumption) (n=6–7). Data reported as means ± SEM. †P < 0.01, *P < 0.05 compared to WT.