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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Acta Physiol (Oxf). 2017 Jun 27;221(3):204–220. doi: 10.1111/apha.12899

Figure 1. TLR4 is expressed in renal vascular smooth muscle cells and LPS treatment increases renal TLR4 expression.

Figure 1

TLR4 is expressed in renal vascular smooth muscle cells, and LPS treatment increases renal TLR4 expression. (a): DAB (TLR4) staining of kidneys from control (left panels) and LPS (1 mg kg−1; i.p.; right panels) treated groups. (b): Data are expressed as a percentage of total kidney area stained for TLR4 from control (white bars) and LPS treatment (grey bars). Each bar represents the mean ± SE. n=4 per group. (c): Immunofluorescence staining of TLR4 in OCT frozen whole kidney sections. Representative images show nuclear staining (blue; Hoechst), vascular smooth muscle α-actin (green), TLR4 (red), and co-localization of TLR4 and vascular smooth α-actin (yellow). Images were taken at 40x magnification. Bar = 20 μm. * P<0.05 vs. control TLR4 expression.