Figure 5.

Nilotinib administration induces leukemia regression in mice. A, Approximately 0.1×10⁶ C1498 cells were injected into C57BL/6 mice through the tail vein. When the white blood cell counts showed the illness, the leukemic mice were treated with vehicle or nilotinib for 3 weeks. External view (left) and quantification of spleen weight (right) from leukemia-bearing mice are shown. Data are presented as mean values ± S.D. B, Photographs (left) are representative external views of livers from leukemia-bearing mice and the graph (right) shows the quantification of liver weight. Data are shown as mean values ± S.D. C, Pictured are images of lung from leukemia-bearing mice. D, Representative images of H&E stained sections of lungs, livers and spleens from leukemia-bearing mice are shown (magnification × 200). E, Wright-Giemsa stained BM cells from leukemia-bearing mice are shown (magnification × 400). F, qPCR was used to determine the expression of DNMT1, DNMT3a, DNMT3b and Sp1 in BM cells from leukemia-bearing mice. G, The genomic DNA in BM cells from leukemia-bearing mice was isolated and subjected to dotblot analysis. H, qPCR was used to determine the expression of p15^INK4B in BM cells from leukemia-bearing mice. Graph shows the quantification of dot intensities. Note, n = 3 mice/group; data are presented as mean values ± S.D; *P < 0.05, **P < 0.01.