Table 4.
Putative resistance genes that were significantly (P < 0.001) associated with responses to races PSTv-14, PSTv-18, PSTv-37, PSTv-40, PSTv-51 and PSTv-125 of Puccinia striiformis f. sp. tritici (Pst) in seedling tests under controlled greenhouse conditions
| Yr genea | Chromb | Confidence interval (cM)c | Tag-SNPd | Allelee | Associated SNPf | R 2 (%)g | To Pst raceh |
|---|---|---|---|---|---|---|---|
| YrTtd-1AS | 1AS | 17.7–21.9 | IWB22778 | C/T | – | 4.3 | PSTv-37 |
| YrTtd-1AL | 1AL | 86.2–90.4 | IWB27332 | C/T | – | 5.2 | PSTv-37 |
| YrTtd-1BS | 1BS | 35.0–39.2 | IWB47025 | C/T | IWA7219, IWB14377, IWB47026, IWB49800, IWB62417, IWB64056 | 4.5–4.8 | PSTv-40 |
| YrTtd-1BL | 1BL | 81.8–86.0 | IWB35698 | A/G | – | 4.6 | PSTv-37 |
| YrTtd-2AL | 2AL | 160.1–164.3 | IWB67229 | C/T | – | 5.8 | PSTv-37 |
| YrTtd-2BS | 2BS | 25.6–29.8 | IWB7081 | C/T | – | 5.7 | PSTv-125 |
| YrTtd - 2BL.1 | 2BL | 138.3–142.5 | IWB48012 | A/G | – | 5.5–6.7 | PSTv-37, PSTv-40, PSTv-51 |
| YrTtd-2BL.2 | 2BL | 163.8–168.0 | IWB59983 | A/G | – | 6.1 | PSTv-125 |
| YrTtd-3AL | 3AL | 108.4–112.6 | IWB71901 | C/T | IWB70903, IWB70904 | 5.5–7.5 | PSTv-51 |
| YrTtd-3BS.1 | 3BS | 84.3–88.5 | IWA7905 | C/T | IWB22863, IWB4227, IWB63776, IWB65606 | 4.5–4.9 | PSTv-40 |
| YrTtd-3BL.1 | 3BL | 95.1–99.3 | IWB37522 | C/T | IWB39508, IWA6510 | 5.1–6.2 | PSTv-37 |
| YrTtd-3BL.2 | 3BL | 147.8–152.0 | IWB59536 | C/T | – | 6.0 | PSTv-51 |
| YrTtd-3BL.3 | 3BL | 185.5–189.7 | IWB10521 | A/G | – | 4.4 | PSTv-40 |
| YrTtd-4AS | 4AS | 23.1–27.3 | IWB55738 | A/G | – | 5.3 | PSTv-40 |
| YrTtd - 4AL.1 | 4AL | 160.7–164.9 | IWA1034 | C/T | – | 4.7–6.9 | PSTv-40, PSTv-51 |
| YrTtd-4BS | 4BS | 30.8–35.0 | IWB56078 | C/T | – | 3.4 | PSTv-18 |
| YrTtd - 4BL.1* | 4BL | 69.9–74.1 | IWA1641 | C/T | – | 4.4–6.6 | PSTv-37, PSTv-125 |
| YrTtd - 4BL.2* | 4BL | 116.7–120.9 | IWB67499 | A/G | – | 5.1–6.3 | PSTv-14, PSTv-37, PSTv-125 |
| YrTtd-5A | 5A | 61.7–65.9 | IWB46475 | A/G | – | 5.8 | PSTv-37 |
| YrTtd-5AL.1 | 5AL | 140.4–144.6 | IWA1829 | A/G | – | 7.8 | PSTv-51 |
| YrTtd-5AL.2 | 5AL | 181.1–185.3 | IWB67141 | A/G | – | 3.9 | PSTv-37 |
| YrTtd-5BS.1 | 5BS | 36.1–40.3 | IWB10728 | C/T | IWB9675 | 4.6–7.0 | PSTv-40 |
| YrTtd-5BS.2 | 5BS | 41.9–46.1 | IWB66991 | C/T | – | 4.3 | PSTv-40 |
| YrTtd-5BL.1 | 5BL | 72.7–76.9 | IWB40681 | A/G | – | 4.3 | PSTv-40 |
| YrTtd-5BL.2 | 5BL | 122.8–127.0 | IWA7733 | A/C | – | 3.2 | PSTv-18 |
| Yr genea | Chromb | Confidence interval (cM)c | Tag-SNPd | Allelee | Associated SNPf | R 2 (%)g | Associated Pst raceh |
|---|---|---|---|---|---|---|---|
| YrTtd - 6AS.1 | 6AS | 9.2–13.4 | IWB63861 | C/T | – | 4.3–4.9 | PSTv-18, PSTv-37, PSTv-40 |
| YrTtd-6AS.2 | 6AS | 19.3–23.5 | IWB63758 | A/C | – | 4.5 | PSTv-40 |
| YrTtd-6AL.1 | 6AL | 91.2–95.4 | IWB9468 | A/G | – | 6.1 | PSTv-37 |
| YrTtd-6AL.2 | 6AL | 114.1–118.3 | IWB72189 | A/G | – | 4.0 | PSTv-37 |
| YrTtd-6BS | 6BS | 69.4–73.6 | IWB60487 | C/T | IWB47211 | 4.6–4.7 | PSTv-40 |
| YrTtd-6BL | 6BL | 74.4–78.6 | IWB12289 | C/T | – | 4.8 | PSTv-14 |
| YrTtd - 7AS* | 7AS | 6.6–10.8 | IWB61392 | C/T | – | 5.1–7.2 | PSTv-14, PSTv-37, PSTv-125 |
| YrTtd-7AL.1 | 7AL | 117.6–121.8 | IWB21459 | A/G | – | 4.6 | PSTv-40 |
| YrTtd-7AL.2* | 7AL | 132.2–136.4 | IWA1944 | A/C | – | 4.9 | PSTv-14 |
| YrTtd-7BS.1 | 7BS | 28.1–32.3 | IWB71730 | C/T | – | 3.9 | PSTv-37 |
| YrTtd-7BS.2 | 7BS | 60.6–64.8 | IWB13775 | A/G | IWA3129, IWB41451, IWB70254, IWB69577 | 5.7–6.2 | PSTv-37 |
| YrTtd-7BL | 7BL | 91.9–96.1 | IWB69807 | A/C | – | 4.5 | PSTv-37 |
a Putative Yr loci that have significant association with seedling response to multiple Pst races are given in bold. Yr loci that have significant association with seedling and field responses are marked by asterisks
b Chromosome positions of putative Yr loci are based on the tetraploid wheat consensus map (Maccaferri et al. 2015a)
c Confidence intervals are determined by confidence intervals of ± 2.1 cM from the peak of the significant associations
d Tag-SNPs are the most significant SNP in the confidence interval of putative genes
e Resistance-associated alleles of tag-SNPs are highlighted in bold
f Significant SNPs associated to the same Pst races as the tag-SNP and falling into the confidence intervals of putative genes
g Phenotypic variations explained by the tag-SNPs
h Pst races that SNP markers are significantly (P < 0.001) associated with