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. 2017 Oct 10;8:1943. doi: 10.3389/fmicb.2017.01943

Table 2.

Comparison of treatments by AMOVA, ANOSIM, and UniFrac, for rumen fungi and protozoa for cows receiving two dietary treatments with or without yeast supplementation under SARA conditions.

Fungal ITS Protozoal 18S
AMOVA ANOSIM Weighted UniFrac AMOVA ANOSIM Weighted UniFrac
P R P W P P R P W P
Location ** 0.13 * 0.65 ** * 0.08 ** 0.87 **
Epimural × Fluid ** 0.05 ns 0.65 ** ** 0.10 * 0.99 **
Epimural × Solid Ta 0.06 ns 0.55 ** * 0.08 * 1 **
Fluid × Solid ** 0.28 ** 0.77 ** * 0.07 * 0.61 **
HF × HG ** 0.93 ** 1 ** ** 0.10 ** 0.65 **
C × Y ns 0.01 ns 0.48 ** ns 0.00 ns 0.61 **
Treatment ** 0.51 ** 0.83 * ** 0.15 ** 0.87 **
HFC × HGC
Epimural ** 0.91 ** 1 ** ns 0.40 * 1 **
Fluid n/a n/a n/a n/a n/a ns 0.00 ns 0.65 **
Solid ** 0.95 ** 1 ** ns 0.11 ns 0.74 **
HFY × HGY
Epimural ** 0.82 ** 1 ** ns 0.31 * 1 ns
Fluid n/a n/a n/a n/a n/a ns 0.19 * 0.5 **
Solid Ta 0.85 T1 1 ** ns 0.00 ns 0.85 **
HFC × HFY
Epimural ns 0.03 ns 0.61 * ns −1.8 ns 0.96 **
Fluid ns 0.01 ns 0.55 * ns 0.03 ns 0.66 **
Solid ns 0.00 ns 0.79 * ns 0.00 ns 0.65 **
HGC × HGY
Epimural ns 0.02 ns 0.74 ** ns 0.31 * 0.95 *
Fluid n/a n/a n/a n/a n/a ns 0.00 ns 0.72 **
Solid ns 0.00 ns 0.63 ** ns 0.02 ns 0.67 **
HFC × HGY
Epimural ** 0.84 ** 1 ** * 0.32 Ta 0.95 *
Fluid n/a n/a n/a n/a n/a ns 0.00 ns 0.53 **
Solid ** 0.84 ** 1 ** ns 0.00 ns 0.74 **
a

Values were significant only before Bonferroni correction.

Diets include high fiber (HF) or high grain (HG), locations include Epimural (E), fluid (F), or solid (S), and treatments include yeast (Y) or Control (C). Significance is determined as P < 0.05,

*

P < 0.001,

**

P > 0.05 (ns), or not enough comparisons to make (n/a). Significance was adjusted by Bonferroni where appropriate.