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. 2017 Oct 10;8:1960. doi: 10.3389/fmicb.2017.01960

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Copyright © 2017 Zheng, Lu, Liang, Jiang, Zhao, Zhan, Liu, Wu and Kang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electron microscopy and RT-PCR. (A) Viral particles purified from field-collected P. striiformis urediniospores. (B) Agarose gel electrophoresis of dsRNAs extracted from purified virus particles of PsVs (lane 1) and the urediospores of P. striiformis from field-collected samples (lane 2). (C) Confirmation of identity of dsRNAs extracted from purified virus particles using PsVs 1-4 specific primers (lanes 1-4, respectively) (Supplementary Table S1). M indicates molecular markers of aaaDNA digested with Hind III.