Table 2.

Porcine models of chronic wounds.

References (year)	Model design								Inoculum				Outcome
	Number of animals	Age (weeks)	Weight (kg)	Sex	Breed	Number of wounds	Infection time	Implant/modification	Bacteria	Dose CFU/ml	Volume	Route	Bacterial verification T/I	Infection stage
Breuing et al., 2003	3	24	45	F	Yorkshire	48	2–47 days	Partial thickness (burn) wounds Wound chamber	S. aureus MRSA 25923	108	1.2 ml	Topical	T: CFU + Histology I: NA	Acute
Davis et al., 2007	6	NR	25–35	F	NR	<279	48 h	Partial thickness wounds Wound dressing	S. aureus ATCC 6,538	107	NR	Topical	T: Histology + SEM + EpiM I: NA	Acute
Hirsch et al., 2008	4	NR	50–60	F	Yorkshire (±diabetic)	56	12 days	Full thickness (diabetic) wounds Wound chamber	S. aureus MRSA 25923	2 × 108	NR	Topical	T: CFU + Histology I: NA	Chronic
Roche et al., 2012	40	NR	20–25	F	Yorkshire crossbred	640–800	21 days	Full thickness wounds Wound dressing	S. aureus MRSA ATCC 33592+PJR006	1 × 107 1 × 108	0.5 or 2 ml	Topical	T: CFU + Histology + SEM I: NA	Chronic
Nusbaum et al., 2012	9	NR	35–40	F	NR	135	23 days	Partial thickness Wound dressing	S. aureus MRSA USA300	106	25 μl	Topical	T: CFU + Histology I: NA	Chronic
Davis et al., 2013	6	NR	40–55	F	NR	36	21 days	Full thickness wounds Wound dressing	P. aeruginosa 6538	500	NR	Topical	T: PCR + CFU I: NA	Chronic

T, verification method in tissue; I, verification method on implant; CFU, colony forming units; SEM, scanning electron microscopy; EpiM, epifluorescence microscopy; NR, not registered; F, female. NA, not available.