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. 2017 Oct 10;8:1942. doi: 10.3389/fmicb.2017.01942

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Copyright © 2017 Zhukova, Sapountzis, Schiøtt and Boomsma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mollicutes bacteria in A. cephalotes across developmental stages. (A,D) Absence of Mollicutes in larval (A) and pupal (D) tissues of A. cephalotes. (B) Gram-negative bacterium in the lumen of a larval gut of A. cephalotes. (C) Higher magnification of the bacterium framed in B. (E) Highly abundant Mollicutes in a worker’s rectum of A. cephalotes (arrows point at some of the bacteria). Frames show bacteria stained with a Mollicutes-specific probe (framed green) and DAPI (framed blue) at higher magnification. (F) Mollicutes-like bacteria lacking a cell wall in the rectum of A. cephalotes worker. Scale bars are 10 μm (A,D,E) and 0.5 μm (B,C,F).