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VLP extraction efficiencies of different buffer-mechanical treatment combinations. Extracted VLP were quantified with epifluorescence microscopy after staining with SybrGold. Asterisks indicate either a too low amount of VLPs or too high background fluorescence for correct counting. Buffers: PC, potassium citrate; SP, sodium pyrophosphate; PBS, phosphate buffered saline; SM, saline magnesium.
