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. Author manuscript; available in PMC: 2018 Sep 17.
Published in final edited form as: Neuroscience. 2017 Jul 17;359:119–129. doi: 10.1016/j.neuroscience.2017.07.020

Figure 5. Co-treatment of isolated DRG neurons with metformin and resveratrol exhibit additive effects on AMPK signaling.

Figure 5

DRG neurons in culture taken from Male ICR mice were treated with vehicle, metformin, or resveratrol alone or metformin + resveratrol for 1 hour at indicated concentrations. A) Phosphorylation of ACC (p-ACC) was assessed by confocal microscopy (green) and neuronal cell nuclei were stained with DAPI (blue). B) Images were processed and the mean fluorescence intensity of pACC was measured and graphed. One-way ANOVA was used to assess differences between the vehicle and treatment groups with Fisher’s LSD post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 (N = 5–12 coverslips per group)