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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: J Biol Chem. 2002 Mar 28;277(24):21922–21929. doi: 10.1074/jbc.M201394200

Fig. 3. Effect of WT and mutant PTPα overexpression on Src in vivo tyrosine phosphorylation and kinase activity.

Fig. 3

Endogenous (Endog) Src was immunoprecipitated from unsynchronized (U) or mitotic (M) non-overexpresser cells (lanes 1 and 2) or from cells overexpressing WT (lanes 3 and 4) or mutant (lanes 5–10) PTPα as indicated. Each immunoprecipitate was divided into aliquots that were used in a kinase assay with [γ-32P]ATP and acid-denatured enolase, followed by electrophoresis and autoradiography of the reaction products (a); immunoblotted with anti-Tyr(P) (Anti-pTyr) antibody (b); immunoblotted with anti-Src antibody (c); or immunoblotted with anti-PTPα antibody (d). SDS-PAGE was performed on 9% gels. The positions of molecular mass markers are indicated in kilodaltons.