Table 5. Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) by Cellic® CTec2 and CBHB.
| Samples | Reducing sugar (mM) | Total reducing sugars (%)c |
|---|---|---|
| (a) OPEFB hydrolysed by CBHBa | 0 | 0 |
| (b) OPEFB hydrolysed by Cellic® CTec2b | 16.9 ± 0.3 | 30.5 ± 0.6 |
| (c) OPEFB hydrolysed by Cellic® CTec2b and CBHBa | 21.5 ± 1.3 | 38.8 ± 2.4 |
| (21.4)d | (38.6)d | |
| Increase in reducing sugar production (%)c | 27.2e | |
Notes.
a
37 µg or 0.0067 U of CBHB was added.
b
2,996 µg or 0.36 FPU of Cellic® CTec2 was added.
c
Total reducing sugar (TRS) was calculated based on Eq. (1).
d
Values predicted by RSM model is bracketed.
e
P < 0.005; a significant increase from (b) to (c).