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The polymerase chain reaction-based SphI restriction fragment length polymorphism (PCR-RFLP) products for C-1562T MMP-9 gene polymorphism on agarose 2%. The PCR products were digested by sphI restriction endonuclease. The CC genotype produced a 435 bp,TT genotype 247bp and 188 bp, and CT genotype 435 bp, 247 bp and 188 bp PCR product.
