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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nature. 2017 May 3;545(7653):238–242. doi: 10.1038/nature22313

Extended Data Fig. 7. Comparative effects of scFv-DKK1c, Wnt3A and scFV-DKK1c-RSPO2 adenoviruses on the intestinal epithelium.

Extended Data Fig. 7

a–c, Mice were treated with adenovirus encoding either scFv-DKK1c or Wnt3A with or without Rspo1/2 or scFv-DKK1c-RSPO2 and tissue was harvested on d4 following treatment. The single chain polypeptide scFv-DKK1c-RSPO2 phenocopies combinatorial treatment with scFv-DKK1c + Rspo. The effects of scFv-DKK1c-RSPO2 were present in a proximal-distal gradient and were confined to the proximal small intestine, in contrast to the effects of scFv-Dkk1c + Rspo1 or scFv-DKK1c + RSPO2 which were pervasive throughout the small intestine. a, H&E of jejunum on D4 post-treatment. b, Ki67 IF. c, CD44 IF. a-c, Bars = 100 mm. d, Lgr5-eGFP-IRES-CreER; Rosa26-tdTomato mice were treated simultaneously with tamoxifen and i.v. adenovirus. Tissue was harvested on d4 post-treatment. Notably, neither treatment with combined scFv-DKK1c + RSPO2 nor the single chain polypeptide scFv-DKK1-RSPO2 alters the lineage tracing of Lgr5+ ISCs compared to RSPO2 alone. Bar = 50 mm.