Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 11;5:73. doi: 10.3389/fchem.2017.00073

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Hellinger, Thell, Vasileva, Muhammad, Gunasekera, Kümmel, Göransson, Becker and Gruber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Structural integrity of the circular peptide probe. (A) Superimposed H^α-chemical shifts of kalata B1 (kB1), [T20K]kB1, and [T20K,W23Bpa]kB1 are shown. (B) Secondary structure elucidation of the cyclotide probe [T20K,W23Bpa]kB1 in comparison to endogenous kB1 and the reference mutant [T20K]kB1 as determined by examining H^α-proton shifts relative to the random coil configuration. Delta (Δ)-values (random coil—experimental values) are presented where numerical values ± 0.1 ppm suggest a well-defined structure. Mutations sites are highlighted in red characters. The recognized H-proton shifts for residues Gly¹¹-Gly¹² and Ser²²-Trp²³ are indicated with gray filled bars. (C) NH-H^α fingerprint region of the TOCSY spectrum of [T20K]kB1 showing intraresidue connectivities. (D) Sequential d_αN(i,i+1) connectivities in the NH-H^α fingerprint region of the NOESY spectrum for [T20K]kB1.