Figure 3.

Biological activity of the circular peptide probe. Bioactivity was assayed by (A) anti-proliferation assays of myelin oligodendrocyte glycoprotein (MOG)-immunized mouse splenocytes and (B) extracellular IL-2 cytokine levels of MOG-activated splenic cells from 2D2 mice. The cyclotide probe [T20K,W23Bpa]kalata B1 (kB1) was measured in comparison to [T20K]kB1 (4 μM each), a MOG-stimulated positive control (+) and an unstimulated cell only control (−). The proliferation data are normalized relative to the MOG-stimulated control. Data are shown as mean ± S.D. of three independent biological experiments. Significance was evaluated using one-way ANOVA with Bonferonni or Dunnett's Post-Hoc test (^*P < 0.05, ^**P < 0.01 and ^***P < 0.001); n.d., not detected.