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. 2017 Oct 11;8:2006. doi: 10.3389/fmicb.2017.02006

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Copyright © 2017 Huang, Cao, Zhou, Huang, Gong, Zhang, Zhu and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Observation of immunofluorescence microscopy for BmAKR in intraerythrocytic B. microti parasites. (A) Observation of the BmAKR recognized by a mouse anti-rBmAKR serum in confocal laser micrographs in mouse iRBC. (B) Control observation of the mouse iRBC not recognized by a mouse normal serum in confocal laser micrographs. (C) Control observation of the normal mouse RBC not recognized by a mouse anti-rBmAKR serum in confocal laser micrographs. FITC, Immunofluorescent staining of B. microti merozoites with mouse serum (green); DAPI, 4′,6′-diamidino-2-phenylindole (DAPI) staining of B. microti merozoite nuclei (blue); BF, Phase-contrast images of B. microti merozoites; MERGE, merged image of FITC, DAPI, and BF; Bars: 2 μm.