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. 2017 Oct 11;8:1754. doi: 10.3389/fpls.2017.01754

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Copyright © 2017 Lamm, Kraner, Hofmann, Börnke, Mock and Sonnewald.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hop/Sti1-knockdown does not affect the unfolded protein response (UPR) in Nicotiana tabacum leaves. (A) Cell death in response to tunicamycin treatment in wild-type and Hop/Sti1-RNAi plants. Leaf disks were floated on a tunicamycin-solution, ion leakage due to cell death was measured as electrical conductivity of the solution. Error bars represent the standard error. (B) Induction of the UPR by PVY^N. Analysis of relative content of spliced bZIP60 by quantitative real-time PCR showed no significant difference (Bonferroni-corrected students t-test, p < 0.05) between wild-type and Hop/Sti1-RNAi plants. Samples were taken from inoculated leaves at the indicated time points. Error bars represent the standard error.