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. 2017 Oct 11;8:1968. doi: 10.3389/fmicb.2017.01968

FIGURE 5.

FIGURE 5

Algicidal effects of silica gel chromatography fractions on cyanobacterial-lawn and high performance liquid chromatography (HPLC) separation of the algicidal fractions S2 and S5. (A–C) indicate the cyanobacterial-lawn results of algicidal fraction S2, S5 and control (other fractions), respectively. HPLC was performed on a Zorbax® Bonus-RP column (4.6 mm × 250 mm, 5 μm). The fraction S2 was eluted with a linear gradient of MeOH/H2O from 5 to 47% (vol/vol) for 60 min at a flow rate of 1.0 ml/min, yielding pure S51107-A (retention time = 47.5–49.0 min) (D). The fraction S5 was purified with MeOH/H2O from 10 to 60% for 40 min yielded pure S51107-B (retention time = 22.6–23.2 min) (E). The arrows denote the algicidal effect of fraction on the cyanobacterial-lawn.