Skip to main content
. 2017 Jul 8;6(10):1313–1320. doi: 10.1016/j.molmet.2017.07.001

Figure 1.

Figure 1

Regulating endogenous Pparγ2 expression and adipogenesis. (A) Pparγ2 sgRNA sequences, distance to transcription start site (TSS) and fold induction. (B) Fold induction of Pparγ2 expression two days post transfection in the C3H/10T1/2-CRISPRa-SAM cells using different sgRNAs binding distinct places on the Pparγ2 promoter. Differentiation of C3H/101/2-CRISPR-SAM cells using Pparγ2 sgRNA or empty vector (EV) stimulated with 5 μM Rosiglitazone or vehicle (DMSO) for 8 days assessed by (C) BODIPY (green) and DAPI (blue) staining or (D) RT-qPCR of the adipogenic markers Ap2 and Adiponectin and Pparγ2. Data are presented as means + SEM, n = 3, One-way ANOVA with Dunnett's test, *p < 0.05 versus EV transfected cells (B) or versus the Pparγ2 sgRNA transfected cells treated with Rosiglitazone (D).