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. 2017 Jul 8;6(10):1313–1320. doi: 10.1016/j.molmet.2017.07.001

Figure 2.

Figure 2

Co-expression of either Prdm16 or Zfp423 with Pparγ2 regulates thermogenic characteristics in adipocytes. C3H/10T1/2-CRISPRa-SAM cells were transfected with EV, Pparγ2 and/or Prdm16/Zfp423 sgRNAs. Expression of (A) Pparγ2, (B) Prdm16, (C) Zfp423, (D) Ap2, and (E) AdipoQ were assessed by RT-qPCR. To stimulate the expression of thermogenic genes, C3H/10T1/2-CRISPRa-SAM cells were treated with 5 μM CL 316,243 for 6 h and (F) Ucp1 and (G) Cidea expression assessed by RT-qPCR. Oxygen consumption was assessed using Seahorse (H). Data are presented as means + SEM, n = 4 (n = 3 for Seahorse data), ANOVA with Dunnett's (A–E) or Sidak-corrected test (H) or Bonferroni-corrected Student's t-tests (F + G), *p < 0.05, **p < 0.01, ***p < 0.001 versus the Pparγ2 sgRNA transfected cells.