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. 2017 Jun 29;6(10):1137–1149. doi: 10.1016/j.molmet.2017.06.014

Figure 3.

Figure 3

Pulldown of ANGPTL3 with ANGPTL8. The conditioned media from cells co-transfected with His-ANGPTL8 and full-length Strep-ANGPTL3 (A), full-length Strep-ANGPTL3 only (B), His-ANGPTL8 and truncated (aa 1-221) Strep-ANGPTL3 (C), or truncated Strep-ANGPTL3 only (D) were bound and eluted from a HisTalon column. Western blots show starting material (SM), column flow-through (FT), column equilibration washes (Equilibration), 10 mM imidazole washes (Wash), and 150 mM imidazole elutions (Elution).