Figure 1.

Generation of mice with mutations in Ffar1. (A) Mutations in Ffar1 induced by ENU in mice. Sperm of mice carrying the mutation 9 and 10 were chosen for in vitro fertilization and generation of mutant mice. (B) Extracellular locations of the mutations 9 and 10 in FFAR1. Note that R258 is located in the agonist-binding domain of the receptor. (C, D, E) Relative mRNA levels in freshly isolated islets from Ffar1^R258W/R258W (black bars) and Ffar1 KO mice (gray bars) compared to their respective wild-type mice expressed as means ± SEM. The number of mice is given in the respective columns. Rps13 was used as housekeeping gene.