Figure 2.

Mutation R258W but not T146S of FFAR1 abrogates palmitate- and TUG-469-induced stimulation of insulin secretion and the effect of palmitate on Ppara mRNA levels. (A, B) Insulin secretion of isolated islets from (A) wild-type littermates (white bars) and Ffar1^R258W/R258W mice (black bars) as well as (B) wild-type littermates (white bars) and Ffar1^T146S/T146S mice (gray bars) measured after 1 h static incubation with substances as indicated. Results are presented as means ± SEM of n = 3–5 independent experiments. (C–F) Isolated islets from wild-type (white bars) and Ffar1^R258W/R258W mice (black bars) were cultured under control condition or exposed to palmitate, 600 μM for 24 h. Relative mRNA levels are expressed as means ± SEM of n = 3–4 independent experiments. Rps13 was used as housekeeping gene. * denotes significance vs respective 2.8 mM glucose or Control (Con). # denotes significance vs. 12 mM glucose, § denotes significance between genotypes.