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. 2017 Aug 1;6(10):1296–1303. doi: 10.1016/j.molmet.2017.07.014

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunostaining of Cl1 and Cl3 markers. A/B. GLU-Venus mouse duodenal sections were immunostained for GFP or proglucagon and PYY, PZP or GPR119 as indicated together with Hoechst stain (blue). C. Percentages of Venus positive cells co-staining for PZP and/or GPR119 as shown in B, calculated from a total of 220 cells counted. D. GLU-Venus mouse duodenal section stained for proglucagon (green) and 5-HT (red) and Hoechst (grey). E. Human duodenal sections co-stained for proglucagon (green), 5-HT (yellow), CASR (red) and Hoechst (blue).