Figure 4.

Impact of high-fat diet (HFD) on glycemia in female ArcPomc^−/− mice. A) Reduced mass of daily food intake in HFD fed WT and ArcPomc^−/− mice relative to their regular chow (RC) fed controls, *P < 0.05 for RC vs. HFD; ^##P < 0.01 for RC ArcPomc^−/− vs. WT (n = 6), 2-way ANOVA followed by Tukey's multiple comparisons test (F_{1, 17} = 3.3, P < 0.05, interaction of genotype and diet); B) No change in daily calorie intake between HFD and RC fed WT or ArcPomc^−/− mice. Increased calorie intake in ArcPomc^−/− mice relative to WT mice, ^∗∗P < 0.01 for ArcPomc^−/− vs. WT (n = 6), 2-way ANOVA followed by Tukey's multiple comparisons test; C) HFD increases body weight in WT and ArcPomc^−/− mice, *P < 0.05 for ArcPomc^−/− RC vs. HFD, ^#P < 0.01 for WT RC vs HFD, 2-way RMANOVA followed by Tukey's multiple comparisons test (F_{24, 112} = 91.6, P < 0.05, interaction of time and genotype); D) No change in fasting blood glucose levels in HFD fed ArcPomc^−/− mice relative to their RC controls, but an increase in fasting blood glucose levels in HFD fed WT mice compared to their RC controls, *P < 0.05 for WT RC vs. HFD, 2-way ANOVA followed by Tukey's multiple comparison test; E) No impairment in glucose tolerance in HFD fed ArcPomc^−/− mice; bar-graph shows AUC for the glucose tolerance test, *P < 0.05 for WT RC vs. HFD, ^##P < 0.05 for ArcPomc^−/− RC or HFD vs WT, 2-way ANOVA followed by Tukey's multiple comparison test; F) Elevated glycosuria in HFD fed ArcPomc^−/− mice. ^∗∗P < 0.01 for ArcPomc^−/− vs. WT, 2-way ANOVA followed by Tukey's multiple comparisons test. KO, ArcPomc^−/− mice; RC, regular chow; HFD, high-fat diet; AUC, area under the curve. Error bars reflect mean ± SEM.